منابع مشابه
Secondary structure of 5 S ribosomal RNA.
A secondary structure model for 16S ribosomal RNA which is based on available chemical, enzymatic, and comparative sequence data shows good agreement between constraints dictated by the model and a wide variety of experimental observations. The four major structural domains created by the base-pairing scheme correspond closely to RNA fragments isolated after nuclease digestion in the presence o...
متن کاملMapping ribosomal protein S20-16 S rRNA interactions by mutagenesis.
Specific ribonucleotides within the 5' domain of Escherichia coli 16 S rRNA were altered by deletion and/or substitution by site-directed mutagenesis of cloned DNA to assess the importance of these particular residues in defining the binding site for ribosomal protein S20. Gel filtration and sucrose gradient centrifugation were employed to measure the binding of ribosomal protein S20 to synthet...
متن کاملHow bacterial ribosomal protein L20 assembles with 23 S ribosomal RNA and its own messenger RNA.
In bacteria, the expression of ribosomal proteins is often feedback-regulated at the translational level by the binding of the protein to its own mRNA. This is the case for L20, which binds to two distinct sites of its mRNA that both resemble its binding site on 23 S rRNA. In the present work, we report an NMR analysis of the interaction between the C-terminal domain of L20 (L20C) and both its ...
متن کاملDirected hydroxyl radical probing of 16 S rRNA using Fe ( II ) tethered to ribosomal protein S 4 ( ribosomal RNA / chemical probing / ribosomes )
Localized hydroxyl radical probing has been used to explore the rRNA neighborhood around a unique position in the structure of the Escherichia coli 30S ribosomal subunit. Fe(II) was attached to ribosomal protein S4 at Cys-31 via the reagent 1-(p-bromoacetamidobenzyl)-EDTA. [FeCys31]S4 was then complexed with 16S rRNA or incorporated into active 30S ribosomal subunits by in vitro reconstitution ...
متن کاملMutations in 16 S Ribosomal RNA and Ribosomal 2 Protein S 5 Associated with High - Level 3 Spectinomycin Resistance in Pasteurella multocida
19 * Corresponding author: Mailing address: Institut für Tierzucht, 20 Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystr. 10, 31535 Neustadt21 Mariensee, Germany. Phone: +49-5034-871-241. Fax: +49-5034-871-246. Electronic 22 mail address: [email protected] 23 AC CE PT ED Copyright © 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved....
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 1991
ISSN: 0022-2836
DOI: 10.1016/0022-2836(91)80182-t